![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3078000:Design
Designed by: Yu Ma Group: iGEM19_Jilin_China (2019-10-02)
HpaX
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 794
Illegal BglII site found at 1260 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 412
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sticky ends of BbsI and BsaI were designed as CTAG and CAAA. So any oligo wanted to be insert should start with CTAG and end with CAAA.
Source
BbsI site+BsaI site+RBS(BBa_J64022)+sfGFP(BBa_K2541400)+double terminator(BBa_B0015)