Coding

Part:BBa_K3078000:Design

Designed by: Yu Ma   Group: iGEM19_Jilin_China   (2019-10-02)
Revision as of 03:32, 3 October 2019 by Registry (Talk | contribs)

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HpaX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 794
    Illegal BglII site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sticky ends of BbsI and BsaI were designed as CTAG and CAAA. So any oligo wanted to be insert should start with CTAG and end with CAAA.


Source

BbsI site+BsaI site+RBS(BBa_J64022)+sfGFP(BBa_K2541400)+double terminator(BBa_B0015)

References