Composite
Part:BBa_K2970003
Designed by: Alexander Bast Group: iGEM19_Hamburg (2019-10-02)
Trigger 1 Composition
Together with Trigger 2, it forms a construct that can open the gate. The affinity between the trigger complex and the gate is greater than that of the gate to itself (loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |