Composite

Part:BBa_K2967017

Designed by: Lijun Bao   Group: iGEM19_NEU_CHINA   (2019-10-02)
Revision as of 15:10, 2 October 2019 by Registry (Talk | contribs)

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Nitrate reporter: PyeaR - Luc composite

In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting to show the result. It has been proved that the expression level can be induced when adding nitric oxide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 193
    Illegal NgoMIV site found at 1537
    Illegal NgoMIV site found at 1558
    Illegal AgeI site found at 1261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1443


[edit]
Categories
Parameters
None