Part:BBa_K2967015

firefly luciferase

Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.

Figure 3. The response to NO of Pyear-luc in E. coli Nissle 1917 . Histogram of Luminescence(RLU): pcdfduet-1 blank, Pyear-luc without SNP, pcdfduet-1 blank, Pyear-luc with 100μM SNP.

[1] Ljung T, Herulf M, Beijer E, et al. Rectal nitric oxide assessment in children with Crohn disease and ulcerative colitis. Indicator of ileocaecal and colorectal affection[J]. Scandinavian journal of gastroenterology, 2001, 36(10): 1073-1076.

Surprisingly, the luciferase in group without SNP was also be activated with undetermined mechanisms. We speculated that it may be caused by leakage expression of promoter PyeaR . Due to this reason, we also constructed an improvement of PyeaR based NO sensor which inserted an extra NOBS downstream of PyeaR to create a ‘roadblocking’ effect. The leakage is reduced by nineteen percent. Please click to “improvement biobricks_” for more details. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]