Part:BBa_K3098024:Design
DSAOP(Designed Anti-oxidant Peptides)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 102
Illegal BamHI site found at 145
Illegal XhoI site found at 343 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of antioxidant peptides isolated from Pinctada fucata meat typically includes 10 to 20 amino acids, which is relatively short and easily degraded by protease or peptidase in Escherichia coli expression systems. Therefore, it is difficult to express these peptides directly. Based on previous reports that an antioxidant peptide from Pinctada fucata meat (amino acid sequence was GAGLPGKRER, named AOP) had high antioxidant activity in vitro, a design containing the GAGLPGKRER sequence was constructed for further expression to obtain a higher yield and improve its stability in our project’s applications in room temperature environment. Meanwhile,and solubility of the designed polypeptide were considered. Finally, The DSAOP DNA sequence encoding 113 amino acids was synthesized and codon-optimized for E. coli expression using Primer Premier 5.0.
Source
Pinctada fucata belongs to the family of pearl oysters from the phylum Mollusca. This species is distributed mainly in the Guangxi, Guangdong, and Hainan provinces of China, the Taiwan Strait, and coastal areas of Japan.
References
[1]Yanyan Wu1*, Yongkai Ma1,2, Laihao Li1, and Xianqing Yang1.Preparation and Antioxidant Activities In Vitro of a Designed Antioxidant Peptide from Pinctada fucata by Recombinant Escherichia coli.J. Microbiol. Biotechnol. (2018), 28(1), 1–11.