Composite

Part:BBa_K2967013

Designed by: Shuang Wu   Group: iGEM19_NEU_CHINA   (2019-09-30)
Revision as of 12:08, 30 September 2019 by Registry (Talk | contribs)

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Amplifier system with hIL-10.

To make sure that amplifier is able to strengthen the expression of gene downstream to promoter hrpL. A coomassie brilliant blue is demonstrated to show that more protein at 35kDA, which should be IL-10, is witnessed when introducing IL-10 gene downstream of promoter hrpL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1292
    Illegal SapI.rc site found at 1925


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Parameters
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