Composite

Part:BBa_K2967012:Design

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-09-30)
Revision as of 11:14, 30 September 2019 by Wangrui (Talk | contribs)


The fixed-gain bio-amplifier


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1312
    Illegal BsaI.rc site found at 3047
    Illegal SapI.rc site found at 1945


Design Notes

In order to quickly test the effect of our amplifier, we used T7 promoter with strong transcriptional activity.


Source

The natural gene regulatory architecture in the hrp control network contains the ingredients of a tunable transcriptional amplifier: The activator proteins HrpR and HrpS form a ultrasensitive high-order co-complex, which bind the upstream activator sequence of the hrpL promoter to remodel the closed σ54 -RNAP-hrpL transcription complex to an open one through ATP hydrolysis.[2]

Figure. The architecture of the fixed-gain amplifier. A fixed-gain transcriptional amplifier is designed on the basis of the transcriptional regulators HrpR (R), HrpS (S) and the promoter hrpL (PhrpL) in the hrp (hypersensitive response and pathogenicity) gene regulatory module from plant pathogen P. syringae.

References