Composite

Part:BBa_K3145003:Design

Designed by: Yazmin Camacho   Group: iGEM19_US_AFRL_CarrollHS   (2019-09-28)
Revision as of 22:54, 29 September 2019 by Yazmin313 (Talk | contribs) (→‎Design Notes)


J18912-bFMO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 240
    Illegal AgeI site found at 263
    Illegal AgeI site found at 567
    Illegal AgeI site found at 897
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


Design Notes

There was an illegal XbaI cut site in between our T7 promoter and RBS region in our pY71-bFMO plasmid that was corrected through site-directed mutagenesis.

Source

N/A

References