Composite
COMP

Part:BBa_K3128033

Designed by: Fahd TIBOURTINE   Group: iGEM19_Grenoble-Alpes   (2019-09-29)
Revision as of 18:47, 29 September 2019 by Fahd (Talk | contribs)


COMP under constitutive promoter

Clickable OmpX is an outer membrane protein whose C and N terminals are in the intracellular domain. It can be used as a scaffold, a non-natural amino acid must be introduced. This can be done through the presence of the stop codon TAG amber in the third loops OmpX.. With a specific tRNA, an azide functionalized amino acid can be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None