Regulatory

Part:BBa_K3142000

Designed by: Linsen Huang   Group: iGEM19_SZPT-CHINA   (2019-09-28)
Revision as of 23:48, 28 September 2019 by Registry (Talk | contribs)

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Acid-induced promoter（PrcfB）

what it is：The secretory signal peptide of the secreted protein usp45 gene of Lactococcus lactis MG1363 and the coding sequence of the first 11 amino acids of the mature peptide. what it does：Effectively secretes proteins from lactic acid bacteria to the outside of the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None