Coding
Part:BBa_K2973019
Designed by: Leandros Tsiotos, Vasiliki Kavvatha Group: iGEM19_Thessaly (2019-09-28)
TEM-optimized beta-lactamase / J23119
Beta-lactamase is an enzyme found widely in the procaryots, and confers resistance to ampicillin. This composite part comprises of the TEM-optimized beta-lactamase CDS (BBa_I757010), regulated by the 119 constitutive Anderson Family Promoter (BBa_J23119) and a universal RBS (BBa_B0034). Transcription is terminated by double terminator (BBa_B0015). This allows for enhanced protein expression in E. coli cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 163
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 783
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Categories
Parameters
None |