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Part:BBa_K2973015:Design

Designed by: Aphrodite Katsaouni, Nick Delkis   Group: iGEM19_Thessaly   (2019-09-28)
Revision as of 19:13, 28 September 2019 by Nickdelkis (Talk | contribs) (→‎References)

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Hepatitis B Virus fragment (281-560bp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose this specific fragment from the HBV genome, due to the availability of specific primer sets in the published literature.


Source

NCBI Hepatitis B Virus Genome, Strain ayw, Accession Number: NC_003977.2

References

Sitnik, R., Paes, Â., Mangueira, C. P., & Pinho, J. R. R. (2010). A real-time quantitative assay for hepatitis B DNA virus (HBV) developed to detect all HBV genotypes. Revista Do Instituto de Medicina Tropical de Sao Paulo, 52(3), 119–124. https://doi.org/10.1590/S0036-46652010000300001

Zauli, D. A. G., Menezes, C. L. P. de, Oliveira, C. L. de, Mateo, E. C. C., & Ferreira, A. C. de S. (2016). In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections. Brazilian Journal of Microbiology, 47(4), 987–992. https://doi.org/10.1016/j.bjm.2016.07.008

Jain, S., Su, Y. H., Su, Y. P., McCloud, S., Xue, R., Lee, T. J., … Hu, C. T. (2018). Characterization of the hepatitis B virus DNA detected in urine of chronic hepatitis B patients. BMC Gastroenterology, 18(1), 19–21. https://doi.org/10.1186/s12876-018-0767-1

Karayiannis, P., Novick, D. M., Lok, A. S. F., Fowler, M. J. F., Monjardino, J., & Thomas, H. C. (1985). Hepatitis B virus DNA in saliva, urine, and seminal fluid of carriers of hepatitis B e antigen. British Medical Journal (Clinical Research Ed.), 290(6485), 1853. https://doi.org/10.1136/bmj.290.6485.1853