Composite

Part:BBa_K2973014:Design

Designed by: Thodoris Kontogiannis   Group: iGEM19_Thessaly   (2019-09-28)
Revision as of 16:34, 28 September 2019 by Tkontogiannis (Talk | contribs) (→‎References)


Trigger for Toehold switch 14 Dictyoglomus turgidum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was designed in order to achieve the transcription of a small part of the 16S rRNA gene of Dictyoglomus kaustophilus. This part behaves as a trigger sequence that will activate the Toehold switch 14 (BBa_K2973012).

Source

Dictyoglomus turgidum strain DSM 6724 16S ribosomal RNA, partial sequence

NCBI Reference Sequence: NR_043385.1

References

Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.

Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002.

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