Composite

Part:BBa_K2973014:Design

Designed by: Thodoris Kontogiannis   Group: iGEM19_Thessaly   (2019-09-28)
Revision as of 16:34, 28 September 2019 by Tkontogiannis (Talk | contribs) (Source)


Trigger for Toehold switch 14 Dictyoglomus turgidum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was designed in order to achieve the transcription of a small part of the 16S rRNA gene of Dictyoglomus kaustophilus. This part behaves as a trigger sequence that will activate the Toehold switch 14 (BBa_K2973012).

Source

Dictyoglomus turgidum strain DSM 6724 16S ribosomal RNA, partial sequence

NCBI Reference Sequence: NR_043385.1

References