Composite

Part:BBa_K2973013:Design

Designed by: Thodoris Kontogiannis   Group: iGEM19_Thessaly   (2019-09-28)
Revision as of 16:22, 28 September 2019 by Tkontogiannis (Talk | contribs) (→‎Source)


Trigger for Toehold switch 13 Geobacillus kaustophilus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was designed in order to achieve the transcription of a small part of the 16S rRNA gene of Geobacillus kaustophilus. This part behaves as a trigger sequence that will activate the Toehold switch 13 (BBa_K2973011).

Source

Geobacillus kaustophilus strain BGSC 90A1 16S ribosomal RNA, complete sequence

NCBI Reference Sequence: NR_115285.2

References