Composite

Part:BBa_K2973012:Design

Designed by: Thodoris Kontogiannis   Group: iGEM19_Thessaly   (2019-09-28)
Revision as of 16:11, 28 September 2019 by Tkontogiannis (Talk | contribs) (→‎References)


Toehold 14 β-Lactamase Dictyoglomus turgidum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This toehold was designed in order to detect the 16S rRNA of the hyperthermophile Dictyoglomus turgidum. The design was made with the NUPACK software http://www.nupack.org.

Source

Dictyoglomus turgidum strain DSM 6724 16S ribosomal RNA, partial sequence

NCBI Reference Sequence: NR_043385.1

References

Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.

Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002.

Qureshi, Sohail A. “β-Lactamase: an Ideal Reporter System for Monitoring Gene Expression in Live Eukaryotic Cells.” BioTechniques, vol. 42, no. 1, 2007, pp. 91–96., doi:10.2144/000112292.

Boehle, Katherine E., et al. “Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.” ACS Sensors, vol. 3, no. 7, 2018, pp. 1299–1307., doi:10.1021/acssensors.8b00163.

http://www.nupack.org