Coding
Cry11Aa(St

Part:BBa_K2938005

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-28)
Revision as of 10:42, 28 September 2019 by Assafi (Talk | contribs)


Cry11Aa + Strep tag

Cry11Aa Bti toxin with Strep-tag attached in the N- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None