Coding
Cry11Aa(St

Part:BBa_K2938005:Design

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-28)
Revision as of 10:40, 28 September 2019 by Registry (Talk | contribs)

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Cry11Aa + Strep tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Strep-tag fused to N- terminal


Source

Gibson assembly

References