Composite

Part:BBa_K3198008

Designed by: Chunyang Song   Group: iGEM19_NUS_Singapore   (2019-09-27)
Revision as of 07:53, 27 September 2019 by Registry (Talk | contribs)

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pBAD-HicB

This part contains the arabinose inducible promoter pBAD and antitoxin component HicB of a type II toxin-antitoxin (TA) system. It functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204
    Illegal BsaI.rc site found at 244


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Categories
Parameters
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