Composite

Part:BBa_K2916018

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-26)
Revision as of 22:51, 26 September 2019 by Konstantinos (Talk | contribs)


Expression of CysRS in E.coli

This part is used for expression of CysRS needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1533
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1087
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 953


[edit]
Categories
Parameters
None