Composite

Part:BBa_K2916044:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-26)
Revision as of 20:52, 26 September 2019 by Konstantinos (Talk | contribs)


Expression of MTF in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1095
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 933
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein was expressed in pET21a in BL21(DE3) E.coli strain.

Source

Sebastian Maerkl Lab at EPFL, Switzerland.

http://www.addgene.org/124133/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.