Composite

Part:BBa_K2992036:Design

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-26)
Revision as of 12:13, 26 September 2019 by YasTen (Talk | contribs) (→‎Design consideration)

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Design considerations

Amplified unmodified from the genome sequences of the host organisms. The ctfA and ctfB genes from the iGEM 2015 entries M36581 BBa_ M36581 and M36582 BBa_ M36582 list the reverse complements of the coding sequences. In our entry we use the same sequences but in the correct 5’-3’ orientation. The RBS for ctfB is contained within the coding sequence for ctfA.