Composite

Part:BBa_K2992035

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-26)
Revision as of 11:39, 26 September 2019 by Registry (Talk | contribs)

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Acetone pathway: csp_Pfdx-5-UTR+RBS-ca_thl-ca_ctfAB-cp_TFdx

Botulinum toxin-predicting acetone production pathway with C. sporogenes Pfdx driving expression of C. acetobutylicum-derived ctfAB, thl and adc.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 569
    Illegal PstI site found at 2136
    Illegal PstI site found at 3401
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2136
    Illegal PstI site found at 3401
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 569
    Illegal PstI site found at 2136
    Illegal PstI site found at 3401
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 569
    Illegal PstI site found at 2136
    Illegal PstI site found at 3401
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None