Coding

Part:BBa_K2916010

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-25)
Revision as of 20:14, 25 September 2019 by Konstantinos (Talk | contribs)


CK protein equipped with a 6x HIS affinity tag


Creatine kinase is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 821
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172


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Categories
Parameters
None