Composite

Part:BBa_K2916038

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-25)
Revision as of 19:25, 25 September 2019 by Konstantinos (Talk | contribs)


Expression of EF-Tu in E.coli


This part is used for expression of EF-Tu needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1292
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 389
    Illegal AgeI site found at 794
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 539


[edit]
Categories
Parameters
None