Composite

Part:BBa_K2916027

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-25)
Revision as of 17:36, 25 September 2019 by Konstantinos (Talk | contribs)


Expression of PheRS in E.coli

This part is used for expression of PheRS needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2533
    Illegal BamHI site found at 140
    Illegal BamHI site found at 1881
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1271
    Illegal AgeI site found at 1264
    Illegal AgeI site found at 2186
    Illegal AgeI site found at 3038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2984
    Illegal SapI.rc site found at 307
    Illegal SapI.rc site found at 2960


[edit]
Categories
Parameters
None