Composite
Part:BBa_K2992030
Designed by: Yaseen Tengur Group: iGEM19_Nottingham (2019-09-25)
Acetone pathway: csp_Pfdx-5-UTR+RBS-ca_thl-cb_ctfAB-cp_TFdx
Botulinum toxin-predicting acetone production pathway with C. sporogenes Pfdx driving expression of ctfAB and theC. acetobutylicum genes thl and adc.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 569
Illegal PstI site found at 3464 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3464
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 569
Illegal PstI site found at 3464 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 569
Illegal PstI site found at 3464 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |