Composite

Part:BBa_K2933273

Designed by: Weisi Wang   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 07:19, 24 September 2019 by Weisi (Talk | contribs)


RBS+Linker h+His+Linker f+MUS-2+T7 terminator

This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+MUS-2) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 877
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This composite part is made up with four basic parts(Tac promoter，RBS a , Linker g , GST+Linker e+MUS-2). It encodes a protein which is MUS-2 fused with His tag. The fusion protein is about 28.3 kD. It is convenient for us to purify our target protein.

Molecular cloning

We used the vector pET28a to construct our expression plasmid.

MUS PCR.png
Figure 1. The PCR result of MUS-2.

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Categories
Parameters
None