Composite

Part:BBa_K2951008:Design

Designed by: YU-RU LIN   Group: iGEM19_CSMU_Taiwan   (2019-09-19)
Revision as of 17:30, 23 September 2019 by Yuruim (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


LysRS-Hemagglutinin fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 275
    Illegal NotI site found at 1256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 864
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1845
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For Hemagglutinin 1.Codon optimization: We optimized the sequence's codon usage for E.coli.

2.RFC 10 compatible: We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.

3.Deletion of transmembrane domain: To improve the solubility of hemagglutinin, we predicted the transmembrane domain with TMHMM Server v. 2.0.


Source

Influenza A virus (A/WSN/1933 TS61(H1N1) obtained from NCBI GenBank(CY010788.1).

References

Yo Han Jang, Seung HeeCho, Ahyun Son, Yun Ha Lee, Jin hee Lee, Kwang-Hee Lee, Baik Lin Seong, High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis, Journal of Virological Methods,Volume 196, February 2014, Pages 56-64 (https://doi.org/10.1016/j.jviromet.2013.10.035)