Composite

Part:BBa_K2933217

Designed by: Pengqian Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 13:39, 23 September 2019 by Pengqian (Talk | contribs) (Usage and Biology=)


T7 promoter+RBS b+Linker h+His+Linker f+VIM-66+T7 terminator

This part consists of T7 promoter, RBS and protein coding sequence(His+Linker f+VIM-66),and the biological module can be built into E.coli for protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 942
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This composite part is made up with seven basic parts, the His tag, T7 promoter, RBS ,Linker h ,Linker f,T7 terminatorand and our target protein VIM-66. It encodes a protein which is VIM-66 fused with His tag. The fusion protein is about 28.3 kD. In order to gain the highly purified target protein, we add His tag in N-terminal of VIM-66 . It is convenient for us to purify our target protein.

Molecular cloning

We insert VIM-66 gene into the standard vector then transfer it into E.coli.

VIM-66-PCR1.jpeg

Figure 1. The result of PCR

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Categories
Parameters
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