Coding
LeuRS

Part:BBa_K2916005

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-24)
Revision as of 12:56, 23 September 2019 by Konstantinos (Talk | contribs)


LeuRS protein equipped with a 6x HIS affinity tag

Leucyl-tRNA synthetase is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1104
    Illegal XhoI site found at 2581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None