Part:BBa_K2933010

subclass B1 metallo-beta-lactamase MUS-2, codon optimized in E. coli

This part encodes a protein called MUS-2, which is a metallo-beta-lactamase of subclass B1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

MUS-2 is a novel variant of the chromosome-encoded β-lactamase MUS-1 in M. odoratimimus, which has been isolated from livestock animals in Lebanon. It could be sure that animals could be a reservoir for such genes and are a source of new and/or emerging multidrug-resistant bacteria and due to possible links with animals, humans may have high risk factors for colonization/infection with such bacteria. Bacteria that carry the enzyme are potentially harmful to humans.

Molecular cloning

We used the vector pGEX-6p-1 to construct our expression plasmid.

Figure 1. Left: The PCR result of MUS-2. Right: The verification results by enzyme digestion.

After verification, it was determined that the construction is successful.

References

[1]Al-Bayssari C, Gupta SK, Dabboussi F, Hamze M, Rolain JM. MUS-2, a novel variant of the chromosome-encoded β-lactamase MUS-1, from Myroides odoratimimus. New Microbes New Infect. 2015 Jun 27;7:67-71. [2] Mammeri H, Bellais S, Nordmann P. Chromosome-encoded beta-lactamases TUS-1 and MUS-1 from Myroides odoratus and Myroides odoratimimus (formerly Flavobacterium odoratum), new members of the lineage of molecular subclass B1 metalloenzymes. Antimicrob Agents Chemother. 2002 Nov;46(11):3561-7.