Coding

Part:BBa_K2933010

Designed by: Wenhui Gong   Group: iGEM19_TJUSLS_China   (2019-09-07)
Revision as of 12:10, 23 September 2019 by Wenhui (Talk | contribs)


subclass B1 metallo-beta-lactamase MUS-2, codon optimized in E. coli

This part encodes a protein called MUS-2, which is a metallo-beta-lactamase of subclass B1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

MUS-2 is a novel variant of the chromosome-encoded β-lactamase MUS-1 in M. odoratimimus, which has been isolated from livestock animals in Lebanon. It could be sure that animals could be a reservoir for such genes and are a source of new and/or emerging multidrug-resistant bacteria and due to possible links with animals, humans may have high risk factors for colonization/infection with such bacteria. Bacteria that carry the enzyme are potentially harmful to humans.

Molecular cloning

We used the vector pGEX-6p-1 to construct our expression plasmid.

TJUSLS China--MUS-2-PCR.png
Figure 1. Left: The PCR result of MUS-2. Right: The verification results by enzyme digestion.

After verification, it was determined that the construction is successful.

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Categories
Parameters
None