Composite
LZCOMP-T18

Part:BBa_K3128028:Design

Designed by: PINERO Lucas   Group: iGEM19_Grenoble-Alpes   (2019-09-23)
Revision as of 07:59, 23 September 2019 by Registry (Talk | contribs)

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COMP fused with Leucine Zipper and T18 subpart of B.Pertussis AC under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 977
    Illegal NgoMIV site found at 1387
    Illegal AgeI site found at 1193
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 179


Design Notes

The signal peptide has a conformation enabling the protein addressing to the membrane. This sequence is cut after the translocation. If the OmpX gene is used without changes, the leucine zipper gene is present before the signal peptide, thus generating a cut in the fusion protein leucine-zipper-OmpX.



Source

pUT18-ZIP plasmid from Euromedex BACTH kit was used (containing the T18 subpart and the leucine zipper gene). OmpX gene and GGS linker were synthesized by IDT because they were unavailable on iGEM plates.


References