Part:BBa_K2957007
hEF1a, IL-8 (CXCL-8)
Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression
What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3667
Illegal PstI site found at 2430
Illegal PstI site found at 2935 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2430
Illegal PstI site found at 2935 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2684
Illegal BamHI site found at 3290
Illegal BamHI site found at 3569
Illegal XhoI site found at 3083 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3667
Illegal PstI site found at 2430
Illegal PstI site found at 2935 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3667
Illegal PstI site found at 2430
Illegal PstI site found at 2935
Illegal NgoMIV site found at 197
Illegal NgoMIV site found at 2818
Illegal AgeI site found at 2025
Illegal AgeI site found at 2196
Illegal AgeI site found at 3673 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2073
Illegal SapI.rc site found at 9
None |