Part:BBa_K3125000

trehalose-6-phosphate synthase from Rhizobium etli CFN 42

otsA gene from R. etli cfn42, with silent mutations. trehalose-6-phosphate synthase.

Usage and Biology

The trehalose-6-phosphate synthase (OtsA or TPS) gene is part of the first step in the most common biosynthetic pathway to create the disacharide, trehalose. The second step is catalyzed by OtsB, also known as trehalose-6-phosphate phosphatase(TPP). OtsA “catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate”(UNIPROT). Trehalose is made in many different biological systems like bacteria and fungi, but is less common in higher eukaryotes. It is important because of its role in protecting organisms against abiotic stress, like drought, cold, heat, desiccation, and salt (Iordachescu and Imai, 2011). Although the mechanism is not fully understood, Rhizobia which overexpress OtsA has been shown to improve the growth and stress tolerance of the legumes in which they colonize (Suarez 2008). OtsA therefore has the potential to be used in improving agriculture practices.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 284
  Illegal XhoI site found at 148
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 373
  Illegal NgoMIV site found at 457
  Illegal NgoMIV site found at 751
  Illegal NgoMIV site found at 935
  Illegal NgoMIV site found at 1102
  Illegal NgoMIV site found at 1357
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 727