Part:BBa_K2926005

TPs1 yeast terminator

Usage and Biology

TPs1 is the natural terminator of the tps1 gen in Saccharomyces cerevisiae, which is encoding the trehalose-6-phosphate synthetase.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Plasmid Design

For the analysis and characterization of the GALL Promotor XXXX in combiantion with the TPs1 Terminaror XXXX, mCherry XXXX was cloned beteen them using Gibson Assembly. The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.

Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.

Characterisation

We followed the standard iGEM protocoll for GFP fluorescence calibration, measuring our cultures against Flourescin. We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
See BBa_K2926073 for the characterization results.