Device

Part:BBa_K145217

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-20)
Revision as of 11:30, 25 August 2008 by Zeunas (Talk | contribs)

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FILTER Complete

FILTER This device was designed to filter out noise in an input signal; in our case p(TetR) PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein.

The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 413
    Illegal NheI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 60


[edit]
Categories
Parameters
None