Coding
otsA

Part:BBa_K3125000:Design

Designed by:   Group: iGEM19_Tacoma_RAINmakers   (2019-09-22)
Revision as of 18:17, 22 September 2019 by Registry (Talk | contribs)

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trehalose-6-phosphate synthase from Rhizobium etli CFN 42


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 373
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 935
    Illegal NgoMIV site found at 1102
    Illegal NgoMIV site found at 1357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 727


Design Notes

Silent mutations made to remove illegal cut sites. kpn1 and hindiii sites added for cloning.


Source

R. etli cfn42

References