Composite
LZOmpX-T18

Part:BBa_K3128026

Designed by: PINERO Lucas   Group: iGEM19_Grenoble-Alpes   (2019-09-22)
Revision as of 17:03, 22 September 2019 by Registry (Talk | contribs)

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OmpX-WT fused with Leucine Zipper and T18 subpart of B.Pertussis AC under constitutive promoter

This biobrick is composed of the external membrane OMPx WT protein fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T18 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under a constitutive promoter allowing the bacteria to have a huge quantity of proteins located at the membrane before the target is added to the external environment (thus, before the detection). Leucine zipper are known to interact by forming homodimers. This biobrick is a part of a positive control for the BACTH assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1008
    Illegal NgoMIV site found at 1418
    Illegal AgeI site found at 1224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 179


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Categories
Parameters
None