![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2933177
Designed by: Weisi Wang Group: iGEM19_TJUSLS_China (2019-09-14)
Tac promoter+RBS a+Linker g+GST+Linker e+ARL-1
This part consists of Tac promoter,RBS and protein coding sequence (GST+Linker e+ARL-1),and the biological module can be built into E.coli for protein expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 181
Usage and Biology
Molecular cloning
First, we used the vector pGEX-6p-1 and the vector to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.
Figure 1. Left: The PCR result of ARL-1. Right: The verification results by enzyme digestion.
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Categories
Parameters
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