![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2933175
Designed by: Weisi Wang Group: iGEM19_TJUSLS_China (2019-09-14)
Tac promoter+RBS a+Linker g+GST+Linker e+VIM-66
This part consists of Tac promoter,RBS and protein coding sequence (GST+Linker e+VIM-66),and the biological module can be built into E.coli for protein expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1532
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 181
Usage and Biology
Molecular cloning
We insert VIM-66 gene into the standard vector then transfer it into E.coli.
Figure 1. Left: The result of PCR, Right:The result of double enzyme digestion verification.LaneM,Marker, Lane1, the plasmid with VIM-66, Lane2, after double enzyme verification
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Categories
Parameters
None |