Part:BBa_I13522

pTet GFP

Untagged GFP behind a constitutive promoter.

Usage and Biology

GFP only control

The version shipped with the 2008 iGEM distribution does not appear to function. Unclear why. Jkm 18:40, 22 August 2008 (UTC)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 724
  

Pictures

• 

  BBa_I13522 visualized under non-UV lightbox

• 

  BBa_I13522 visualized under 254nm wavelength UV lightbox

Characterization of Cell-Free Chassis

This part was used to characterize the cell-free chassis. Details of characterization can be found on the cell-free characterization page. Other interesting isothermal properties of this construct are available at the [http://2007.igem.org/Imperial/Cell_by_Date/Testing Cell By Date project page] of Imperial 2007 wiki.

Parameter	Value and Description
Optimal Temperature	Expression increases with temperature from 4°C to 30°C
Optimal DNA Concentration	Works optimally with 2µg of DNA in 60 µl of cell-extract
Peak Expression Time	Peak rate of output occurs after approximately 85 minutes
Expression Lifespan	Expression levels off after 2 hours
Expression Capacity	Can synthesize over 200pmols of GFPmut3b in 6 hours