![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3182300
Designed by: Oliver Hild Walett Group: iGEM19_Linkoping_Sweden (2019-07-15)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 91
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
pT7-mNG
This is a improved variant of BBa_K2671000. The biobrick codes for mNeonGreen (mNG), which is a fluorescent protein with great intensity. The protein is currently ranked third in intensity, only beaten by mVenus-Q69M (basic) and Skylan-S (photoswitchable).
This part has a T7-system as well as a 5’-UTR region, instead of the AraC-pBAD system present in the non-improved biobrick BBa_K2671000. By using the T7-DNA-directed-RNA-polymerase (T7-DdRNAP) from a bacteriophage over the native DNA-directed-RNA-polymerase (n-DdRNAP) native in E. coli the expression is greatly increased. This part requires a host carrying the T7-DdRNAP, such as E. coli BL21 (DE3) Gold which was the chassi we used.
![](/wiki/images/8/82/T--Linkoping_Sweden--mngimprovementsequence.png)
Figure 1. A schematic representation of the improvement. Where the promotor (purple, pBAD) of the previous biobrick (BBa_K2671000) has been exchanged to pT7. Translation enhancing '5-UTR containing g10-L RBS (BBa_K1758100) has been added as well to the improvement. Together with removal of extra bases (named UTR in figure 1) which is non-coding. A BamHI site was added after the His-tag. The blue colors illustrate the protein coding part of the biobricks.
Usage and Biology
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Categories
Parameters
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