Part:BBa_K3182300

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 91
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

pT7-mNG

This is a improved variant of BBa_K2671000. This part codes for mNeonGreen, which is a fluorescent protein with great intensity. mNeonGreen has a excitation maximum at 506 nm and a emission maximum at 517, providing a small Stoke's shift. It's color can be seen under normal white light as a yellow-green color. This part includes a T7 promotor as well as a 5'-UTR region for higher expression.

Figure 1. A schematic representation of the improvement. Where the promotor (purple, pBAD) of the previous biobrick (BBa_K2671000) has been exchanged to pT7. Translation enhancing '5-UTR containing g10-L RBS (BBa_K1758100) has been added as well to the improvement. Together with removal of extra bases (named UTR in figure 1) which is non-coding. A BamHI site was added after the His-tag. The blue colors illustrate the protein coding part of the biobricks.

Usage and Biology

Figure 2.