Regulatory

Part:BBa_K2963030

Designed by: Experiment group members   Group: iGEM19_JNU-China   (2019-09-19)
Revision as of 10:38, 19 September 2019 by Registry (Talk | contribs)

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Tac Promoter (including two operator genes(lacO))


This promoter is a hybrid of two operons: the trp and lac operons. This promoter is inducible by IPTG and commonly used in Corynebacterium glutamicum for overproduction of proteins. Corynebacterium glutamicum constitutively produces the LacIq protein, a strong pTAC promoter repressor which can bind with lac operator. In our experiment, we add two lac operators in order to forbid leaked expression of downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None