Composite
PLac_LZ-

Part:BBa_K3128023:Design

Designed by: PINERO Lucas   Group: iGEM19_Grenoble-Alpes   (2019-09-18)
Revision as of 21:36, 18 September 2019 by Melo (Talk | contribs) (→‎Source)


OmpX-WT with Leucine Zipper and T25 subpart of Bordetella Pertussis AC under lactose promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 344


Design Notes

The signal peptide has a conformation enabling the protein addressing to the membrane. This sequence is cut after the translocation. If the OmpX gene is used without changes, the leucine zipper gene is present before the signal peptide, thus generating a cut in the fusion protein leucine-zipper-OmpX.


Source

pKT25-ZIP plasmid from Euromedex BACTH kit was used (containing the T25 subpart and the leucine zipper gene). OmpX gene and GGS linker were synthesized by IDT because they were unavailable on iGEM plates.

References