Coding
TmEncH

Part:BBa_K3111003:Design

Designed by: Matas Deveikis   Group: iGEM19_UCL   (2019-09-18)
Revision as of 21:02, 18 September 2019 by Matas deveikis (Talk | contribs) (→‎Design Notes)


T. maritima encapsulin (6-His) from BBa_K2686002 (no stop codon)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457


Design Notes

Stop codon removed at the end of BBa_K2686002 BioBrick to allow fusion at the C-terminus of the protein.

Source

Enterokinase site is taken from Novagen plasmid pET-51b.

References