Composite

Part:BBa_K3128019

Designed by: PINERO Lucas   Group: iGEM19_Grenoble-Alpes   (2019-09-18)
Revision as of 18:11, 18 September 2019 by Registry (Talk | contribs)

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COMP fused with T18 subpart of Bordetella Pertussis AC under constitutive promoter

This biobrick is composed of the external membrane OMPx muted protein fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T18 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under constitutive promoter allowing the bacteria to have a huge quantity of proteins located at the membrane before the target is added to the external environment (thus, before the detection).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1300
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 841
    Illegal NgoMIV site found at 1251
    Illegal AgeI site found at 1057
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/nominee
//cds/membrane
//cds/membrane/extracellular
//cds/membrane/receptor
//classic/signalling/sender
//rbs/prokaryote/constitutive
Parameters
sender