Intermediate

Part:BBa_K3128012

Designed by: Melodie Dupre   Group: iGEM19_Grenoble-Alpes   (2019-09-18)
Revision as of 17:06, 18 September 2019 by Registry (Talk | contribs)

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OmpX-WT fused with T25 subpart of Bordetella Pertussis adenylate cyclase under lactose promoter

This biobrick is composed of the external membrane OMPx WT protein fused at its N-terminal end with a 54 aa GGS linker (BBa_K3128010) followed by the T25 subunit of the Bordetella Pertussis adenylate cyclase. This biobrick is under plac promoter in order to be activated when the cAMP messenger is present in the system.

iGEM Grenoble-Alpes designed this biobrick thanks to the pKNT25 plasmid contained in the Euromedex BACTH kit. More informations in the design page. It is an intermediate because the team want to change the plac promoter with a constitutive promoter. It allows the bacteria to have a huge quantity of proteins located in the membrane before the target is added to the external environment (thus, before the detection).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1636
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None